borosilicate glass chamber slides labtek 155411 Search Results


ethynyl  (Thermo Fisher)
99
Thermo Fisher ethynyl
Subcellular detection of HIV-1 reverse-transcription and pre-integration complexes (RTC/PIC). MDMs from four donors in three independent experiments were infected with 100 ng of CA of R5-pseudotyped HIV-1 NL4-3 (IN.eGFP) in the presence of 10 µM <t>5-ethynyl-2′-deoxyuridine</t> (EdU). At 24 h p.i., medium was changed and further viral entry was blocked by 5 µM MVC. Cells were fixed at 24 ( A ), 36 ( B ), 48 ( C ), and 60 h p.i. ( D ), click-labeled, and immunostained with anti-CA antiserum (cyan); the nuclear envelope was marked by immunostaining with anti-laminA/C antibody (white). EdU and IN.eGFP signals in the merged panels are represented in red and green, respectively. Exemplary complexes of different types are indicated by arrowheads: solid arrows indicate co-localizing EdU and IN.eGFP signals; empty arrows identify HIV-1 complexes lacking EdU signals. Scale bars represent 5 µm. ( E ) Proportion of IN.eGFP-positive objects in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.002; n.s.: not significant. ( F ) Proportion of IN.eGFP- and EdU-positive objects from ( E ) in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.005; * p = 0.022; n.s.: not significant.
Ethynyl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/borosilicate+glass+chamber+slides+labtek+155411/pmc06266216-99-38-40?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
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biofilms  (Fisher Scientific)
86
Fisher Scientific biofilms
Subcellular detection of HIV-1 reverse-transcription and pre-integration complexes (RTC/PIC). MDMs from four donors in three independent experiments were infected with 100 ng of CA of R5-pseudotyped HIV-1 NL4-3 (IN.eGFP) in the presence of 10 µM <t>5-ethynyl-2′-deoxyuridine</t> (EdU). At 24 h p.i., medium was changed and further viral entry was blocked by 5 µM MVC. Cells were fixed at 24 ( A ), 36 ( B ), 48 ( C ), and 60 h p.i. ( D ), click-labeled, and immunostained with anti-CA antiserum (cyan); the nuclear envelope was marked by immunostaining with anti-laminA/C antibody (white). EdU and IN.eGFP signals in the merged panels are represented in red and green, respectively. Exemplary complexes of different types are indicated by arrowheads: solid arrows indicate co-localizing EdU and IN.eGFP signals; empty arrows identify HIV-1 complexes lacking EdU signals. Scale bars represent 5 µm. ( E ) Proportion of IN.eGFP-positive objects in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.002; n.s.: not significant. ( F ) Proportion of IN.eGFP- and EdU-positive objects from ( E ) in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.005; * p = 0.022; n.s.: not significant.
Biofilms, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/borosilicate+glass+chamber+slides+labtek+155411/pmc08595767-485-0-13?v=Fisher+Scientific
Average 86 stars, based on 1 article reviews
biofilms - by Bioz Stars, 2026-07
86/100 stars
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polystyrene plates  (Corning Life Sciences)
90
Corning Life Sciences polystyrene plates
Subcellular detection of HIV-1 reverse-transcription and pre-integration complexes (RTC/PIC). MDMs from four donors in three independent experiments were infected with 100 ng of CA of R5-pseudotyped HIV-1 NL4-3 (IN.eGFP) in the presence of 10 µM <t>5-ethynyl-2′-deoxyuridine</t> (EdU). At 24 h p.i., medium was changed and further viral entry was blocked by 5 µM MVC. Cells were fixed at 24 ( A ), 36 ( B ), 48 ( C ), and 60 h p.i. ( D ), click-labeled, and immunostained with anti-CA antiserum (cyan); the nuclear envelope was marked by immunostaining with anti-laminA/C antibody (white). EdU and IN.eGFP signals in the merged panels are represented in red and green, respectively. Exemplary complexes of different types are indicated by arrowheads: solid arrows indicate co-localizing EdU and IN.eGFP signals; empty arrows identify HIV-1 complexes lacking EdU signals. Scale bars represent 5 µm. ( E ) Proportion of IN.eGFP-positive objects in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.002; n.s.: not significant. ( F ) Proportion of IN.eGFP- and EdU-positive objects from ( E ) in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.005; * p = 0.022; n.s.: not significant.
Polystyrene Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/borosilicate+glass+chamber+slides+labtek+155411/10__2310_slash_7290__2008__00023-39-7-18?v=Corning+Life+Sciences
Average 90 stars, based on 1 article reviews
polystyrene plates - by Bioz Stars, 2026-07
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lsm 880 inverted confocal microscopy  (Carl Zeiss)
90
Carl Zeiss lsm 880 inverted confocal microscopy
Subcellular detection of HIV-1 reverse-transcription and pre-integration complexes (RTC/PIC). MDMs from four donors in three independent experiments were infected with 100 ng of CA of R5-pseudotyped HIV-1 NL4-3 (IN.eGFP) in the presence of 10 µM <t>5-ethynyl-2′-deoxyuridine</t> (EdU). At 24 h p.i., medium was changed and further viral entry was blocked by 5 µM MVC. Cells were fixed at 24 ( A ), 36 ( B ), 48 ( C ), and 60 h p.i. ( D ), click-labeled, and immunostained with anti-CA antiserum (cyan); the nuclear envelope was marked by immunostaining with anti-laminA/C antibody (white). EdU and IN.eGFP signals in the merged panels are represented in red and green, respectively. Exemplary complexes of different types are indicated by arrowheads: solid arrows indicate co-localizing EdU and IN.eGFP signals; empty arrows identify HIV-1 complexes lacking EdU signals. Scale bars represent 5 µm. ( E ) Proportion of IN.eGFP-positive objects in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.002; n.s.: not significant. ( F ) Proportion of IN.eGFP- and EdU-positive objects from ( E ) in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.005; * p = 0.022; n.s.: not significant.
Lsm 880 Inverted Confocal Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/borosilicate+glass+chamber+slides+labtek+155411/pmc09502984-76-35-35?v=Carl+Zeiss
Average 90 stars, based on 1 article reviews
lsm 880 inverted confocal microscopy - by Bioz Stars, 2026-07
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Image Search Results


Subcellular detection of HIV-1 reverse-transcription and pre-integration complexes (RTC/PIC). MDMs from four donors in three independent experiments were infected with 100 ng of CA of R5-pseudotyped HIV-1 NL4-3 (IN.eGFP) in the presence of 10 µM 5-ethynyl-2′-deoxyuridine (EdU). At 24 h p.i., medium was changed and further viral entry was blocked by 5 µM MVC. Cells were fixed at 24 ( A ), 36 ( B ), 48 ( C ), and 60 h p.i. ( D ), click-labeled, and immunostained with anti-CA antiserum (cyan); the nuclear envelope was marked by immunostaining with anti-laminA/C antibody (white). EdU and IN.eGFP signals in the merged panels are represented in red and green, respectively. Exemplary complexes of different types are indicated by arrowheads: solid arrows indicate co-localizing EdU and IN.eGFP signals; empty arrows identify HIV-1 complexes lacking EdU signals. Scale bars represent 5 µm. ( E ) Proportion of IN.eGFP-positive objects in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.002; n.s.: not significant. ( F ) Proportion of IN.eGFP- and EdU-positive objects from ( E ) in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.005; * p = 0.022; n.s.: not significant.

Journal: Viruses

Article Title: Detailed Characterization of Early HIV-1 Replication Dynamics in Primary Human Macrophages

doi: 10.3390/v10110620

Figure Lengend Snippet: Subcellular detection of HIV-1 reverse-transcription and pre-integration complexes (RTC/PIC). MDMs from four donors in three independent experiments were infected with 100 ng of CA of R5-pseudotyped HIV-1 NL4-3 (IN.eGFP) in the presence of 10 µM 5-ethynyl-2′-deoxyuridine (EdU). At 24 h p.i., medium was changed and further viral entry was blocked by 5 µM MVC. Cells were fixed at 24 ( A ), 36 ( B ), 48 ( C ), and 60 h p.i. ( D ), click-labeled, and immunostained with anti-CA antiserum (cyan); the nuclear envelope was marked by immunostaining with anti-laminA/C antibody (white). EdU and IN.eGFP signals in the merged panels are represented in red and green, respectively. Exemplary complexes of different types are indicated by arrowheads: solid arrows indicate co-localizing EdU and IN.eGFP signals; empty arrows identify HIV-1 complexes lacking EdU signals. Scale bars represent 5 µm. ( E ) Proportion of IN.eGFP-positive objects in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.002; n.s.: not significant. ( F ) Proportion of IN.eGFP- and EdU-positive objects from ( E ) in n cells in three subcellular localizations at different time points. Numbers of detected objects are given in parentheses. Proportions were compared using a two-tailed Z -test (α = 0.05); *** p < 0.0001; ** p < 0.005; * p = 0.022; n.s.: not significant.

Article Snippet: To visualize HIV-1 reverse-transcription and pre-integration complexes (RTC/PIC), MDMs were seeded in eight-well LabTek chamber slides (#155411, Thermo Scientific, Waltham, MA, USA) and infected with 100 ng of CA of HIV-1 (IN.eGFP) in complete RPMI containing 10 µM 5-ethynyl-2´-deoxyuridine (EdU; Thermo Scientific).

Techniques: Infection, Labeling, Immunostaining, Two Tailed Test